Endocrine Abstracts

The anabolic role of androgens in growth and regeneration, has similar implications in periodontal tissues. Studies suggest that androgens and growth factors could affect activity of the enzyme alkaline phosphatase, being closely linked to anabolic effects on tissues matrices. Nicotine has detrimental effects on healing. One of the mechanisms involved is impairment of alkaline phosphatase activity. The aim of this investigation is to study the response of nicotine to platelet ...

Physiologically active androgens and growth factors contribute to healing responses by a variety of mechanisms, including stimulation of enzymes associated with matrix turnover. Some of these effects may be partially negated by nicotine. Both androgens and growth factors are able to compensate for oxidative effects induced by nicotine, being a significant deterrent to wound healing. The aim of this investigation is to establish the metabolic yields of androgen biomarkers in re...

The aim of this investigation is to establish the effect of alkaline phosphatase on androgen metabolism in human gingival fibroblasts (obtained local Ethical Committee approval) and its possible mediation by the alkaline phosphatase inhibitor levamisole and nicotine, using radiolabelled testosterone as substrate. Monolayer cultures of fibroblasts were established in Eagle's MEM. Duplicate incubations were performed in multiwell plates with 14C-testosterone as substrate, in the...

Phenytoin (Ph) causes gingival overgrowth in inflamed gingivae. Androgen metabolism could be influenced by alkaline phosphatase activity and inhibited by the alkaline phosphatase inhibitor levamisole (L). In view of the anabolic role of the androgen metabolite 5alpha dihydrotestosterone (DHT), the aim of this investigation is to study the effects of Ph, histamine (H) and the alkaline phosphatase inhibitor levamisole (L) on the metabolism of androgen substrates by human gingiva...